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sbf scanning electron microscopy  (Carl Zeiss)


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    Carl Zeiss sbf scanning electron microscopy
    Sbf Scanning Electron Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sbf scanning electron microscopy/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    sbf scanning electron microscopy - by Bioz Stars, 2026-05
    90/100 stars

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    sbf scanning electron microscopy datasets  (Visage Imaging GmbH)
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    Immunohistochemistry reveals much more MelanA positive cells within the LCs than within the non-crypt rich limbal areas (A, B). Serial-block face scanning electron micrographs showing the edge of one limbal crypt (C, D). After manual segmentation and 3D reconstruction (E, F, G) putative LESC (smallest epithelial cell with a high nucleus/cytoplasm ratio) is represented in green, melanocyte (dendritic cell containing electron dense granules) in red, limbal stromal cell in yellow. Blue volumes correspond to nuclei. Continuous line in G corresponds to block face represented in C. Dashed line in G corresponds to block face represented in D. Scale bars: 50 μm (A, B) and 7 μm (C, D).
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    Immunohistochemistry reveals much more MelanA positive cells within the LCs than within the non-crypt rich limbal areas (A, B). Serial-block face scanning electron micrographs showing the edge of one limbal crypt (C, D). After manual segmentation and 3D reconstruction (E, F, G) putative LESC (smallest epithelial cell with a high nucleus/cytoplasm ratio) is represented in green, melanocyte (dendritic cell containing electron dense granules) in red, limbal stromal cell in yellow. Blue volumes correspond to nuclei. Continuous line in G corresponds to block face represented in C. Dashed line in G corresponds to block face represented in D. Scale bars: 50 μm (A, B) and 7 μm (C, D).

    Journal: PLoS ONE

    Article Title: Localisation of Epithelial Cells Capable of Holoclone Formation In Vitro and Direct Interaction with Stromal Cells in the Native Human Limbal Crypt

    doi: 10.1371/journal.pone.0094283

    Figure Lengend Snippet: Immunohistochemistry reveals much more MelanA positive cells within the LCs than within the non-crypt rich limbal areas (A, B). Serial-block face scanning electron micrographs showing the edge of one limbal crypt (C, D). After manual segmentation and 3D reconstruction (E, F, G) putative LESC (smallest epithelial cell with a high nucleus/cytoplasm ratio) is represented in green, melanocyte (dendritic cell containing electron dense granules) in red, limbal stromal cell in yellow. Blue volumes correspond to nuclei. Continuous line in G corresponds to block face represented in C. Dashed line in G corresponds to block face represented in D. Scale bars: 50 μm (A, B) and 7 μm (C, D).

    Article Snippet: SBF scanning electron microscopy datasets were converted into voxels (volumetric picture elements) and areas of interest manually segmented and reconstructed into a 3D volume using Amira (Visage Imaging Inc.).

    Techniques: Immunohistochemistry, Blocking Assay